Part:BBa_K4025003

CBP (Chitin Binding Protein)

CBP is a protein from Vibrio cholerae that is codon-optimised for E. coli. CBP is found in the periplasm where it can bind to chitin oligosaccharides and the periplasmic region of ChiS for activation. In the absence of chitin oligosaccharides, CBP binds to and inactivates ChiS by favoring phosphorylation of its receiver domain.

CBP was transformed into plasmid backbone pSB1k02 and a growth curve was constructed to measure cell burden. See: https://2021.igem.org/Team:Lubbock_TTU/Contribution.

As can be seen from the growth curves, the L1 transcriptional unit with CBP did not overly burdened cell growth– all transformed cells survived. It should be noted; however, that the OD600 readings of all cultures were not standardized prior to inoculation, seen by the varying y-intercepts of all growth curves. Because of this, this data should not be used as quantitative measurements of growth kinetics, but rather a qualitative demonstration of relative growth.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]